Given the valid remarks about the discussion becoming a bit technical, if I can make an observation about this thread, I don't think the opening post actually made it terribly clear quite what the scope of the topic was and, just what the learning experience was intended to be. That's not a criticism, but I think it is easy enough to understand that a simple question can lead to a complex answer, and from that point things just escalate quite quickly.
I assume the aim of the thread was to introduce DOF, but in so doing it has raised several things and they in turn have lead to other discussions. While that is all good, I can see how quickly the thread can lose the plot and become unwieldy and confusing. For eaxmple, the instructions were to shoot at two apertures and note the other alterations required to the camera settings and also the outcome in the image. Well, let's look at the results so far....
Changing aperture has an impact on DOF. It also has an impact on one or more of shutter speed, ISO, lighting. In addition it can raise questions about lens IQ and also diffraction. Since most of that is wrapped up in the topic of exposure, this could easily lead on to other things like exposure accuracy, metering, optimising exposure, exposing for raw vs JPEG. Ouch!
Shutter speed has consequences for shake and blur and raises questions about tripods, image stabilisation and acceptable shutter speeds related to focal length.
Changing ISO has an impact on noise, but it is not a straightforward impact, since ISO alone is not the reason for noise.
There are probably further consequences too - at one point we got side tracked into talking about filters, and then there was the comment about choosing/adjusting focal point depending on DOF.
Of course, while there are some broad brush rules about DOF, like stopping down increases it, there is much more to it than that, influenced by camera format, focal length, subject distance, aperture, final viewing size and final viewing distance.
All in all it's quite a massive subject, with all manner of inter-related consequences when you make just one small change to something. Going forward, I'm not sure what the best way might be to constrain each thread to the learning point that was intended, but perhaps a little more clarity on the goals and scope of each thread, set out at the beginning might help.