In the present case, we've seen that the actual amount of DOF is quite small. So, we're going to have to use a tape measure to see where the limits lie in our subject. Hang on, though - where exactly are we measuring from? The front of the lens? The film plane? A lens nodal plane (which one, and how on earth do we know whereabouts in the lens mount we should be measuring from)? Is the size of the circle of confusion appropriate for our intended degree of enlargement and viewing distance?
Hence, I tend to be very skeptical about the practical use of this type of calculation, except insofar as it gives a feel for approximate distances and quantities. The only exception I might make personally would be cases where an exact reproduction ratio was required in technical work (which I don't do).