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IS/VR is designed to counteract moment if there is not movement to counteract it will still be trying to and as such this was actually introduce movement into the image :bang:
The newer implementations of Canon's IS system (lenses introduced after the EF300mm f/2.78L IS USM in 1999) will detect that that the camera is on a tripod and takes measures to prevent that happening, so you don't need to switch IS off when using a tripod.
http://cpn.canon-europe.com/content/education/technical/image_stabilization_lenses.do?page=2