Wow. So many people all saying the same thing, and they're all wrong. I'm sorry to have to break it to you, folks, but the old 1/3 - 2/3 rule is an old wives' tale based on an inadequate understanding of the maths of DOF.
A little while ago I had a slow day in the office so I did some calculations. (Well actually
DOFMaster did.) Suppose you have a 55mm lens on a 350D at f/8. The hyperfocal distance is 20m. With the subject at various distances, the DOF is as follows:
* Subject at 1m: DOF from 0.95m to 1.05m (48% in front)
* Subject at 2m: DOF from 1.82m to 2.22m (45% in front)
* Subject at 3m: DOF from 2.61m to 3.52m (43% in front)
* Subject at 5m: DOF from 4m to 6.65m (38% in front)
* Subject at 6.67m: DOF from 5m to 10m (33% in front)
* Subject at 10m: DOF from 6.67m to 20m (25% in front)
* Subject at 15m: DOF from 8.57m to 60.2m (12% in front)
* Subject at 19m: DOF from 9.73m to 395.4m (2% in front)
Note that there is only one point at which the DOF is 1/3 in front of the subject and 2/3 behind the subject, and that is when the subject is at exactly 1/3 of the hyperfocal distance.