I don't know to be honest
@PhotoVanGogh.
I've got a pretty powerful laptop so it can handle the 140 files but looking at the images I added to the macro forum that were 35/40 I don't think there is a need. It's just a bit of trial and error.
I did the first one at 140 images, Step count of 1, F2.8 and ISO 100 in Ap mode.
Then I played around and took three sets for the second (amber) stone / mineral. I altered F stop to 5.6, the starting AF placement and then played with 30/35 images but altered the step from 2-4. It was the final set that I used for the stack in affinity (35/40 images at step 4).
It doesn't take long to actually take the images and my laptop spat these out quickly, couple of minutes, so worst case if it doesn't look right I can do it again and increase the image count.
Plan to play around more today and I think the number of objects and distance between front object and rear object will come into play...further away, the more images you'll need (or steps between them) I'm guessing.
But, it's genuinely all new to me. This was the first time I've done this using the camera focus Bracketing. I've done stacking before (typically stacked Exposure bracketed shots) but not this.
To be honest I only stumbled on this setting after watching a YT video lol